The new DeltaEmerald Single-Box Laser System from APE. This system allows simultaneous SRS imaging of two vibrational bands with its revolutionary dual-color SRS (DC-SRS) scheme. The DeltaEmerald is aimed at users at the forefront of SRS microscopy. It provides the best signal-to-noise performance and the capability of background subtraction as well as simultaneous imaging of two vibrational bands
The new deltaEmerald allows simultaneous SRS imaging of two vibrational bands with its revolutionary dual color SRS (DC-SRS) scheme. It is aimed at users at the forefront of SRS microscopy, giving the best signal-to-noise performance and the capability of background subtraction as well as simultaneous imaging of two vibrational bands
Two Stokes pulses, separated by 85 cm-1 and modulated at different frequencies are overlapped with the tunable Pump pulse. The pulse length of about 1 ps, 10 cm-1 … 15 cm-1 bandwidth and several 100 mW output power in each beam are ideal parameters for Coherent Raman imaging.
The pulses of all three beams are shot noise limited due to solid state laser design and proven OPO technology. The shot noise limit of -162 dBc/Hz above 1 MHz (limited by the noise measurement setup with 4.8 mA) allows for fast image acquisition and superior signal-to-noise ratio.
Fully automated tuning, power control and temporal and spatial overlap of all three beams are included.
Additionally a ~100 fs output at 1030 nm is provided for efficient SHG and two-photon imaging.
Specifications
| Wavelength Stokes 1 | 1034 nm |
| Wavelength Stokes 2 | 1025 nm |
| Wavelength Pump (OPO Signal) | 740 nm … 990 nm |
| ∆v Pump – Stokes | 350 cm-1 … 3800 cm-1 |
| Power Stokes 1 | > 300mW (unmodulated), >150 mW modulated (20 MHz) |
| Power Stokes 2 | > 300mW (unmodulated), >150 mW modulated (13 MHz) |
| Power Pump (OPO Signal) | > 400 mW at 800 nm |
| Spectral bandwidth Pump and Stokes beams | 10 cm-1 … 15 cm-1 |
| Pulse width | 1 ps |
| Repetition rate | 80 MHz |
| Noise | Shot noise limited (-162 dBc/Hz) > 1 MHz (Pump and Stokes ) |
Beam Parameters
| Pointing Stability | < 100 µrad per 100 nm |
| M2 | < 1.2 (Pump and Stokes) |
| Ellipticity | < 20% |
| Polarization | Linear; horizontal > 100:1 |
| Beam divergence | 0.9 (± 0.2) mrad (at 800 and Stokes) |
| Beam waist diameter | 1.3 (± 0.2) mm at 800 nm; 1.7 (± 0.2) mm Stokes beams |
Additional Femtosecond Output
| Wavelength | 1030 nm ± 3 nm |
| Power | 300 mW |
| Pulse width | < 200 fs |
| Beam divergence | < 1.5 mrad |
Features
| Integrated modulators for Stokes beams | Built-in EOM with resonant fixed frequency of 20 MHz and 13 MHz |
| Power attenuators | Integrated for Pump and Stokes, independent for each beam |
| Delay management | Automated compensation for microscope dispersion |
| Remote control | Ethernet TCP/IP |
Mechanical dimensions (L x W x H) / weight:
| deltaEmerald | 961 mm x 781 mm x 277 mm (see drawing for details) // 130 kg |
| Laser control unit | 19 inch / 4U // 10 kg |
| Chiller | 19 inch / 4U // 14 kg |
Further requirements:
| Power consumption | 100 V … 240 V, 50 Hz … 60 Hz, max. 10 A (at 100 V) |
| Operating temperature | 20°C … 25°C, air conditioning (stability ± 1°C) recommended |
| Relative humidity | < 60% |
https://www.ape-berlin.de/en/dc-srs/#specification
Dual-colour SRS imaging
Simultaneous imaging of CH2 & CH3 for nondestructive and fast Coherent Raman histology
Background removal of non-resonant artefacts
Study of drug penetration, metabolic imaging, microplastics discrimination, plant imaging, cell imaging, fingerprint imaging etc.
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Photonic Solutions Ltd
Unit 2.2 Quantum Court
Heriot-Watt University Research Park
Edinburgh
EH14 4AP
