The new DeltaEmerald Laser System from APE for dual-colour SRS microscopy. The DeltaEmerald allows simultaneous SRS imaging of two vibrational bands with its revolutionary dual-color SRS (DC-SRS) scheme. Users at the forefront of SRS microscopy get the best signal-to-noise performance and the capability of background subtraction as well as simultaneous imaging of two vibrational bands
The new deltaEmerald allows simultaneous SRS imaging of two vibrational bands with its revolutionary dual color SRS (DC-SRS) scheme. It is aimed at users at the forefront of SRS microscopy, giving the best signal-to-noise performance and the capability of background subtraction as well as simultaneous imaging of two vibrational bands
Two Stokes pulses, separated by 85 cm-1 and modulated at different frequencies are overlapped with the tunable Pump pulse. The pulse length of about 1 ps, 10 cm-1 … 15 cm-1 bandwidth and several 100 mW output power in each beam are ideal parameters for Coherent Raman imaging.
The pulses of all three beams are shot noise limited due to solid state laser design and proven OPO technology. The shot noise limit of -162 dBc/Hz above 1 MHz (limited by the noise measurement setup with 4.8 mA) allows for fast image acquisition and superior signal-to-noise ratio.
Fully automated tuning, power control and temporal and spatial overlap of all three beams are included.
Additionally a ~100 fs output at 1030 nm is provided for efficient SHG and two-photon imaging.
| Wavelength 1 (Stokes 1) | 1034nm |
| Wavelength 2 (Stokes 2) | 1025nm |
| Wavelength 3 (Pump-Signal) | 740 nm … 990 nm |
| ∆v Pump – Stokes | 350 cm-1 … 3800 cm-1 |
| Power Stokes 1 | >300 mW (unmodulated), >150 mW modulated (20 MHz) |
| Power Stokes 2 | >300 mW (unmodulated), >150 mW modulated (13 MHz) |
| Power Pump (Signal) | >400 mW at 800 nm |
| Spectral bandwidth | 10 cm-1 … 15 cm-1 (Pump and Stokes) |
| Pulse width | 1 ps |
| Repetition rate | 80 MHz |
| Noise | Shot noise limited (-162 dBc/Hz) >1 MHz for Pump and Stokes |
| Beam Parameters | |
|---|---|
| Pointing Stability | <100 µrad per 100 nm |
| M | <1.2 for Pump and Stokes |
| Ellipticity | <20% |
| Polarization | Linear, horizontal >100 : 1 |
| Beam divergence | 0.9 mrad ± 0.2 mrad at 800 nm and Stokes |
| Beam waist diameter | 1.3 mm ± 0.2 mm at 800 nm, 1.7 mm ± 0.2 mm for Stokes |
| Additional femtosecond output | |
| Wavelength | 1030 nm ± 3 nm |
| Power | 300 mW |
| Pulse width | <200 fs |
| Beam divergence | <1.5 mrad |
| Features | |
| Integrated modulator for Stokes | Built-in EOMs with resonant fixed frequency of 20 MHz and 13 MHz |
| Power attenuators | Integrated for Pump and Stokes, independent for each beam |
| Delay management | Automated compensation for microscope dispersion |
| Connectivity | Ethernet and Software interface TCP/IP |
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Dual-colour SRS imaging
Simultaneous imaging of CH2 & CH3 for nondestructive and fast Coherent Raman histology
Background removal of non-resonant artefacts
Study of drug penetration, metabolic imaging, microplastics discrimination, plant imaging, cell imaging, fingerprint imaging etc.
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Photonic Solutions Ltd
Unit 2.2 Quantum Court
Heriot-Watt University Research Park
Edinburgh
EH14 4AP
