The DCS-120 MACRO FLIM System system scans objects directly in the focal plane of the scanner. Image objects up to 15mm at a resolution of about 25 micrometres. The DCS-120 MACRO uses excitation by ps diode lasers, fast scanning by galvanometer mirrors, confocal detection, and FLIM by Becker & Hickl‘s multidimensional TCSPC technique to record fluorescence lifetime images at high temporal resolution, high spatial resolution, and high sensitivity.
The DCS-120 MACRO is a complete macro laser scanning FLIM system that can scan fields up to 15 mm in diameter with pixel numbers from 16 x 16 to 2048 x 2048. It can convert existing conventional microscopes into fully functional confocal laser scanning microscopes with TCSPC detection.
Unlike conventional confocal systems, the DCS-120 MACRO system does not require an expensive laser scanning microscope. Instead, the scan head is attached to the side or backport of a conventional inverted microscope.
DCS-120 MACRO includes a fast preview function, interactive scanner control, easy selection of scan field, and beam-stop function for single-point measurements. Recording of FLIM or steady-state fluorescence images simultaneously in two fully parallel wavelength channels, laser wavelength multiplexing, multi-wavelength FLIM, time-series FLIM, ultra-fast time-series recording by temporal-mosaic FLIM, spatial mosaic FLIM, Z stack FLIM, phosphorescence lifetime imaging (PLIM), fluorescence lifetime-transient scanning (FLITS) and FCS recording.
Data analysis is performed by BH’s new SPCImage NG FLIM analysis software. SPCImage NG is a combination of time-domain and phasor analysis. By running the calculations on a GPU (Graphics Processor Unit) the results are available within seconds. Other features are the availability of image segmentation via the phasor plot or via 2D time-domain histograms, and automatic modelling of the system IRF. Repeated recalibration by recording the actual IRF is thus unnecessary.
System Components
| Available Wavelengths | 375 nm, 405 nm, 440 nm, 473 nm, 488 nm, 510 nm |
| Repetition rates | 20 MHz, 50 MHz, 80 MHz, CW |
| Pulse width | typ. 60 ps |
| Intensity control, electronic | 1:10 |
| Beam correction optics | Beam-profile and astigmatism correction |
| Fibre coupling | Single-mode, into Point-Source fibre |
| Power delivered into fibre: | 405 nm: 1 mW, 440 nm: 0.4 mW, 473 nm: 0.4 mW, 488 nm: 0.4 mW |
| Multiplexing | Pixel by pixel, line by line, or frame by frame |
| Beam blanking | During x and y flyback, via bh GVD-120 scan controller |
Molecular Imaging with FLIM
Fluorescence Correlation – FCS
Simultaneous metabolic Imaging and pO2 imaging
Cellular and tissue fluorescence
In-vivo FLIM of Skin
Multiwavelength FLIM
Simultaneous FLIM/PLIM
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Photonic Solutions Ltd
Unit 2.2 Quantum Court
Heriot-Watt University Research Park
Edinburgh
EH14 4AP
